Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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ChIP-seq

(Protocol summary only for purposes of this preview site)

An alternative to the microarray-based detection methods is direct sequencing of the ChIP-enriched DNA (ChIP-seq). In the ChIP-seq protocol, the ChIP DNA is analyzed using next-generation sequencing platforms (notably the Illumina Genome Analyzer; see Chapter 11). Millions of short sequence reads are obtained from direct sequencing of the ChIP DNA, and the densities of recovered ChIP sequence reads along the genome are used to determine the binding sites of the protein. Although a much smaller amount of ChIP DNA is required for ChIP-seq, the current sequencing platforms still require amplification of the ChIP DNA by ligation-mediated PCR. Owing to its digital nature, ChIP-seq has become the standard method for genome-wide ChIP analysis.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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